Cells and gene expression programs in the adult human heart

Monika Litvinukova; Carlos Talavera-Lopez; Henrike Maatz; Daniel Reichart; Catherine L. Worth; Eric L. Lindberg; Masatoshi Kanda; Krzysztof Polanski; Eirini S. Fasouli; Sara Samari; Kenny Roberts; Elizabeth Tuck; Matthias Heinig; Daniel DeLaughter; Barbara McDonough; Hiroko Wakimoto; Joshua M. Gorham; Emily Nadelmann; Krishnaa T. Mahbubani; Kourosh Saeb-Parsy; Giannino Patone; Joseph J Boyle; Hongbo Zhang; Hao Zhang; Anissa Viveiros; Gavin Oudit; Omer Bayraktar; J. G. Seidman; Christine Seidman; Michela Noseda; Norbert Hubner; Sarah A. Teichmann

We identified six subpopulations of aCM, indicating considerable heterogeneity ( Figure 2B ) particularly between the right and left chambers. Notably, HAMP , a master regulator of iron homeostasis, was significantly enriched in over 50% of RA CM compared to 3% LA CM ( Supplementary Table B4 ) 29 , consistent with prior studies of RA tissues 8 and confirmed by smFISH ( Figure 2G ). HAMP has unknown roles in cardiac biology, but Hamp-null mice have electron transport chain deficits and lethal cardiomyopathy 30 . The RA enrichment of HAMP may imply energetic differences between right and left aCM. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.03.024075 doi: bioRxiv preprint aCM2 cells were more prominent in the RA than LA. Consistent with this, aCM 2 cells had the highest proportion (65% , Supplementary Table B3 ) The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.03.024075 doi: bioRxiv preprint (green) and CNN1 (red) i. Nuclei were DAPI-stained (dark blue). Scale bar 10µm.