Evaluation of Measles Vaccine Virus as a Vector to Deliver Respiratory Syncytial Virus Fusion Protein or Epstein-Barr Virus Glycoprotein gp350

Mok, Hoyin; Cheng, Xing; Xu, Qi; Zengel, James R; Parhy, Bandita; Zhao, Jackie; Wang, C. Kathy; Jin, Hong

RSV-specific antibody responses were measured by RSV F-specific ELISA and microneutralization assay. sF1 or sF3 induced RSV F-specific IgG antibody in the vaccinated cotton rats (about 15.5-15.9 log 2 post dose 1 and 22.1-23.7 log 2 post dose 2). The RSV F-specific IgG antibody titers were similar between the sF1 and sF3 vaccinated groups. RSV neutralizing antibody titers were also measured against RSV A2 strain. After the first dose, sF1 and sF3 induced RSV neutralizing Ab and titers were boosted to 7.5-log 2 and 8.9-log 2 after the second dose ( Table 1) . There was no statistical difference in antibody response between sF1 and sF3 vaccinated animals and those intranasally infected with 2 doses of RSV A2 strain (9.4 log 2 ± 1.5, data not shown). Animals vaccinated with UV-inactivated sF3 did not have detectable RSV antibody (data not shown), indicating that virus immunogenicity was dependent on live virus infection.