Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Document date: 2019_3_18
ID: 6nq7y1qe_28
Snippet: To investigate the effects of C3P3-G1 enzyme expression on viability and cytotoxicity, HEK-293 cells were transfected with the pCMV-NP868R-(G 4 S) 2 -K1ERNAP(R551S) plasmid with or without the pK1E(G)-Luciferase gene reporter plasmid. Empty backbone plasmids were used as controls. Cells were analyzed at selected time points using the MultiTox-Glo Multiplex Cytotoxicity Assay (Promega), a sequential-reagent-addition fluorescent and luminescent ass.....
Document: To investigate the effects of C3P3-G1 enzyme expression on viability and cytotoxicity, HEK-293 cells were transfected with the pCMV-NP868R-(G 4 S) 2 -K1ERNAP(R551S) plasmid with or without the pK1E(G)-Luciferase gene reporter plasmid. Empty backbone plasmids were used as controls. Cells were analyzed at selected time points using the MultiTox-Glo Multiplex Cytotoxicity Assay (Promega), a sequential-reagent-addition fluorescent and luminescent assay that measures the relative number of live and dead cells in cell populations.
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