Characterization of the budding compartment of mouse hepatitis virus: evidence that transport from the RER to the Golgi complex requires only one vesicular transport step

We then used glucose 6 phosphatase as a cytochemical 10 nm gold) and antibodies against the COOH terminus of the M protein (6 nm gold, arrows). Note that most of the virions (/arge arrowhead) and the membranes enclosing them label with both markers. In B, the RER (ribosome indicated by arrow) labels weakly for the M protein but there is no significant labeling for the lectin. The structure containing the virions (/arge arrowhead) is strongly labeled for both markets. In C and D the rough ER again contains variable amounts of M protein (indicated) using the COOH-terminal antibodies, but essentially no labeling for HPA. In contrast, the structures containing the virions, which in C are probably continuous with the RER, are strongly labeled for both markets. A ribosome (R) on the RER is indicated. N, nucleus. Bars, 100 rim.