Author: Jorge Blazquez-Prieto; Covadonga Huidobro; Ines Lopez-Alonso; Laura Amado-Rodriguez; Paula Martin-Vicente; Cecilia Lopez-Martinez; Irene Crespo; Cristina Pantoja; Pablo J Fernandez-Marcos; Manuel Serrano; Jacob I Sznajder; Guillermo M Albaiceta
Title: Cellular senescence limits acute lung injury induced by mechanical ventilation Document date: 2020_3_25
ID: ebwxryai_24
Snippet: Lung adenocarcinoma A549 cells were cultured in elastic plates (Bioflex culture plates, Flexcell int.) in presence of DMEM supplemented with 10% FBS and stretched for 24 hours (20% deformation, 20 cycles per minute). Non-stretched cells cultured in the same plates were used as control. After 120 hours, abundance of senescence-associated (SA)-b-galactosidase, Tp53 and Cdkn1a expression and cell cycle distribution were studied (see below)......
Document: Lung adenocarcinoma A549 cells were cultured in elastic plates (Bioflex culture plates, Flexcell int.) in presence of DMEM supplemented with 10% FBS and stretched for 24 hours (20% deformation, 20 cycles per minute). Non-stretched cells cultured in the same plates were used as control. After 120 hours, abundance of senescence-associated (SA)-b-galactosidase, Tp53 and Cdkn1a expression and cell cycle distribution were studied (see below).
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