Selected article for: "freshly prepared solution and room temperature"
Author: Jorge Blazquez-Prieto; Covadonga Huidobro; Ines Lopez-Alonso; Laura Amado-Rodriguez; Paula Martin-Vicente; Cecilia Lopez-Martinez; Irene Crespo; Cristina Pantoja; Pablo J Fernandez-Marcos; Manuel Serrano; Jacob I Sznajder; Guillermo M Albaiceta
Title: Cellular senescence limits acute lung injury induced by mechanical ventilation
  • Document date: 2020_3_25
    • ID: ebwxryai_28
    Snippet: Senescence-associated b-galactosidase staining . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.24.005983 doi: bioRxiv preprint A549 cells were washed twice with PBS and fixed in 0.25% (v/v) glutaraldehyde and 2% (v/v) formaldehyde in PBS for 15 minutes at room temperature. Following fixation, cells were.....
    Document: Senescence-associated b-galactosidase staining . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.24.005983 doi: bioRxiv preprint A549 cells were washed twice with PBS and fixed in 0.25% (v/v) glutaraldehyde and 2% (v/v) formaldehyde in PBS for 15 minutes at room temperature. Following fixation, cells were washed 3 times with PBS and incubated overnight at 37°C in freshly-prepared staining solution at pH 6 containing 1mg/ml 5-bromo-4-chloro-3indolyl-ß-D galactoside (X-Gal), 40 mM citric acid/sodium phosphate pH 6, 5 mM K3Fe(CN)6, 5 mM K4Fe(CN)6, 150 mM NaCl, and 2 mM MgCl2, as previously described (18) . After staining, cells were rinsed in PBS 3 times and stored in PBS at 4°C until image collection.

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