Title: Endoplasmic reticulum localization of Sec12p is achieved by two mechanisms: Rer1p-dependent retrieval that requires the transmembrane domain and Rer1p-independent retention that involves the cytoplasmic domain Document date: 1996_7_2
ID: 45x96b5d_7
Snippet: Saccharomyces cerevisiae strains used in this study are listed in Table I . Yeast cells were grown in YPD [1% (wt/vol) Bacto yeast extract (Difco Laboratories Inc., Detroit, MI), 2 % (wt/vol) polypeptone (Nihon Seiyaku, Tokyo, Japan), and 2% (wt/vol) glucose] or in MVD [0.67% yeast nitrogen base without amino acids (Difco Laboratories) and 2% glucose] medium supplemented appropriately. MCD medium is MVD containing 0.5% casamino acids (Difco Labor.....
Document: Saccharomyces cerevisiae strains used in this study are listed in Table I . Yeast cells were grown in YPD [1% (wt/vol) Bacto yeast extract (Difco Laboratories Inc., Detroit, MI), 2 % (wt/vol) polypeptone (Nihon Seiyaku, Tokyo, Japan), and 2% (wt/vol) glucose] or in MVD [0.67% yeast nitrogen base without amino acids (Difco Laboratories) and 2% glucose] medium supplemented appropriately. MCD medium is MVD containing 0.5% casamino acids (Difco Laboratories Inc.).
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