Comparison Of Four Anti-Avian IgY Secondary Antibodies Used In Western Blot And Dot-Blot ELISA To Detect Avian Bornavirus Antibodies In Four Different Bird Species

Escandon, Paulina; Heatley, J Jill; Berghman, Luc R; Tizard, Ian; Musser, Jeffrey MB

A weak signal was detected for ABV antibodies in positive Blue and gold macaw plasma when using antiduck IgY secondary antibody, however this signal was similar to that detected in the negative Blue and gold macaw, positive and negative Cockatiel, and negative Mallard plasma. A strong signal was detected for ABV antibodies in positive Mallard plasma when using antiduck IgY secondary antibody. A weak signal was detected when anti-chicken IgY secondary antibody was used with positive and negative Blue and gold macaw, positive and negative Cockatiel, and negative Mallard plasma. A strong signal was detected for ABV antibodies in positive Mallard plasma when using anti-chicken IgY secondary antibody.