Selected article for: "absence presence and additional marker"
Title: Compartmentation of the Golgi complex: brefeldin-A distinguishes trans- Golgi cisternae from the trans-Golgi network
  • Document date: 1990_9_1
    • ID: 47k2yobm_29
    Snippet: We also used the man6P receptor as an additional marker to test whether transient occupants of the TGN return to the ER in BFA. CHO cells were metabolically labeled and chased for 4 h to permit newly synthesized man6P receptors to achieve their steady-state distribution, primarily in endosomes, the plasma membrane, and the TGN (7, 15, 16, 22) . The reversible inhibitor of Golgi tx-mannosidase I, dMM (4), was included in the labeling and chase med.....
    Document: We also used the man6P receptor as an additional marker to test whether transient occupants of the TGN return to the ER in BFA. CHO cells were metabolically labeled and chased for 4 h to permit newly synthesized man6P receptors to achieve their steady-state distribution, primarily in endosomes, the plasma membrane, and the TGN (7, 15, 16, 22) . The reversible inhibitor of Golgi tx-mannosidase I, dMM (4), was included in the labeling and chase media, so that man6P receptors within and beyond the TGN would bear high-mannose oligosaccharides. Next, dMM was washed away, and cells were incubated for various lengths of time in the presence or absence of BFA. We then monitored the potential galactosylation of man6P receptors that would occur if they returned to the ER.

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