Title: Compartmentation of the Golgi complex: brefeldin-A distinguishes trans- Golgi cisternae from the trans-Golgi network Document date: 1990_9_1
ID: 47k2yobm_15
Snippet: Cell surface man6P receptor oligosaccharides were labeled with UDP-[3H]Gal (Amersham Corp.) and galactosyltransferase according to Duncan and Kornfeld (10) . Cells were then incubated at 37°C (in 100 ml suspension culture) for 0 or 6 h in the presence or absence of 10 ttg/rnl BFA. Man6P receptors were isolated by pentamannosyl phospbate-Sepharose chromatography (34) . Purified receptors were digested with pmnase (CalBiochem-Behring Corp., La Jol.....
Document: Cell surface man6P receptor oligosaccharides were labeled with UDP-[3H]Gal (Amersham Corp.) and galactosyltransferase according to Duncan and Kornfeld (10) . Cells were then incubated at 37°C (in 100 ml suspension culture) for 0 or 6 h in the presence or absence of 10 ttg/rnl BFA. Man6P receptors were isolated by pentamannosyl phospbate-Sepharose chromatography (34) . Purified receptors were digested with pmnase (CalBiochem-Behring Corp., La Jolla, CA), and the resulting glycollptides were subjected to jack bean/5-galactosidase digestion and Sephadex (}-25 chromatography to assess sialic acid acquisition (10).
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