Detection and genetic characterization of Mamastrovirus 5 from Brazilian dogs

Alves, Christian D.B.T.; Budaszewski, Renata F.; Torikachvili, Marcela; Streck, André F.; Weber, Matheus N.; Cibulski, Samuel P.; Ravazzolo, Ana P.; Lunge, Vagner R.; Canal, Cláudio W.

Phylogenetic inferences were also carried out with the partial and complete sequences of OFR2 at nucleotide and amino acid levels. The partial genome sequences of MAstV5 obtained in the present study and those available in GenBank, together with selected Mamastrovirus reference sequences from other species, generated two evolutionary trees (Fig. 2) . Forty-three reference strains and the four sequences from this study corresponding to 19 Mamastrovirus species were delineated with high bootstrap support throughout the entire tree ( Fig. 2A) . In the MAstV5 clade, all of the present sequences clustered with "Gillingham/2012/UK" (GenBank accession number NC 026814), although with low amino acid identities of approximately 80% (Table 2) . Consequently, these four new sequences grouped within Chinese sequences, suggesting a different genotype putatively named as MAsTV5a (Fig. 2B) . Through a pairwise comparison of the ORF2 nucleotide sequence, a high degree of nucleotide identity, ranging from 95% to 99%, was detected among the Brazilian type strains of this study. The study sequences showed a closer relationship with the Chinese strains grouping in genotype a. More (Fig. 2B ).