Title: Coronavirus induction of class I major histocompatibility complex expression in murine astrocytes is virus strain specific Document date: 1994_9_1
ID: 4bf8eiix_13
Snippet: Class I expression was also evaluated by FACS | using an indirect immunoftuorescent staining procedure. Briefly, single cell suspensions of astrocytes were adjusted to 105 to 106 cells/tube before the addition of class 1-specific primary antibodies. FITC-labeled goat anti-mouse IgG F(ab)2 was used as secondary antibody (Jackson ImmunoResearch Laboratories, West Grove, PA). Fluorescence data were collected on 3 x 103 to 104 viable cells, indicated.....
Document: Class I expression was also evaluated by FACS | using an indirect immunoftuorescent staining procedure. Briefly, single cell suspensions of astrocytes were adjusted to 105 to 106 cells/tube before the addition of class 1-specific primary antibodies. FITC-labeled goat anti-mouse IgG F(ab)2 was used as secondary antibody (Jackson ImmunoResearch Laboratories, West Grove, PA). Fluorescence data were collected on 3 x 103 to 104 viable cells, indicated by forward light scatter intensity, using a FACStar cell sorter (Becton Dickinson & Co.). Again, background fluorescence was determined in cells stained in the absence of primary antibody.
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