Bioinformatics Study on Structural Protein of Severe Acute Respiratory Syndrome Coronavirus 2 (Sars-Cov-2) For Better Understanding the Vaccine Development

Sumaira Gulzar; Saqib Hussain

Coronaviruses, a genus belonging to the Coronaviridae family, contain the largest genome (26.4 kb to 31.7 kb) of RNA viruses with a diameter of 120-160 nm, consisting of a single-stranded positivesense RNA molecule (de Groot et al., 2012) The broad genome has given this family of viruses extra plasticity in their adaptation and alteration of genes. The G + C content of coronavirus genomes ranges from 32% TO 43% The genome consists of seven genes arranged in sequence [5′-replicase ORF1ab, spike (S), envelope (E), membrane (M), nucleocapside (N)-3′] with small untranslated regions in both termini and additional ORFs in each subgroup of coronavirus. (Woo et al., 2009) 5 'Non-structural protein coding regions consisting of two-thirds genome replicase genes 1 and structural and non-essential gene. 2-7 regions consisting of structural and non-essential protein coding regions [5] . The genes are translated from genomic mRNA 2 -7. Subgenomic RNAs encode the main Surface Proteins (S), Envelope Protein (E), Membranes Protein (M), and Nucleocapside Protein (Ng et al., 2003) Surface protein of SARS CoV-2 join ACE2 (angiotensin-converting enzyme2) and to infect cells.