Author: Abes, Rachida; Moulton, Hong M.; Clair, Philippe; Yang, Sung-Tae; Abes, Said; Melikov, Kamran; Prevot, Paul; Youngblood, Derek S.; Iversen, Patrick L.; Chernomordik, Leonid V.; Lebleu, Bernard
Title: Delivery of steric block morpholino oligomers by (R-X-R)(4) peptides: structure–activity studies Document date: 2008_9_16
ID: 5j496cx0_21
Snippet: Exponentially growing HeLa pLuc705 cells (1.75 Â 10 5 cells seeded and grown overnight in 24-well plates) were co-incubated with the (R-Ahx-R) 4 -PMO conjugates and with 20 mg/ml saponin for 30 min. The conjugates were removed and the cells were washed twice with PBS and incubation continued for 24 h in complete medium (DMEM plus 10% FBS). Cells were washed twice with ice-cold PBS, lysed with Reporter Lysis Buffer and processed as described abov.....
Document: Exponentially growing HeLa pLuc705 cells (1.75 Â 10 5 cells seeded and grown overnight in 24-well plates) were co-incubated with the (R-Ahx-R) 4 -PMO conjugates and with 20 mg/ml saponin for 30 min. The conjugates were removed and the cells were washed twice with PBS and incubation continued for 24 h in complete medium (DMEM plus 10% FBS). Cells were washed twice with ice-cold PBS, lysed with Reporter Lysis Buffer and processed as described above.
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