Author: Bancroft, Tara; DeBuysscher, Blair L.; Weidle, Connor; Schwartz, Allison; Wall, Abigail; Gray, Matthew D.; Feng, Junli; Steach, Holly R.; Fitzpatrick, Kristin S.; Gewe, Mesfin M.; Skog, Patrick D.; Doyle-Cooper, Colleen; Ota, Takayuki; Strong, Roland K.; Nemazee, David; Pancera, Marie; Stamatatos, Leonidas; McGuire, Andrew T.; Taylor, Justin J.
Title: Detection and activation of HIV broadly neutralizing antibody precursor B cells using anti-idiotypes Document date: 2019_10_7
ID: 63yvpuqx_50
Snippet: For all experiments, 0.25 µl Fixable Viability Dye eFluor 506 or eFluor 780 (eBioscience) or Fixable Viability Stain 700 (BD Biosciences) was included in the surface antibody cocktail to distinguish live from dead cells. Flow cytometry was performed on a five-laser (355 nm, 405 nm, 488 nm, 561 nm, and 640 nm) LSR II, LSRFortessa, FACSARIA II, or FACSymphony device (BD Biosciences) and analyzed with FlowJo 10 software (Tree Star). 2 × 10 4 fluor.....
Document: For all experiments, 0.25 µl Fixable Viability Dye eFluor 506 or eFluor 780 (eBioscience) or Fixable Viability Stain 700 (BD Biosciences) was included in the surface antibody cocktail to distinguish live from dead cells. Flow cytometry was performed on a five-laser (355 nm, 405 nm, 488 nm, 561 nm, and 640 nm) LSR II, LSRFortessa, FACSARIA II, or FACSymphony device (BD Biosciences) and analyzed with FlowJo 10 software (Tree Star). 2 × 10 4 fluorescent AccuCheck counting beads (Thermo Fisher Scientific) were added to the samples before flow cytometry and used to calculate total numbers of cell subtypes in the columnbound and flow-through suspensions, as previously described .
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