Detection and activation of HIV broadly neutralizing antibody precursor B cells using anti-idiotypes

Bancroft, Tara; DeBuysscher, Blair L.; Weidle, Connor; Schwartz, Allison; Wall, Abigail; Gray, Matthew D.; Feng, Junli; Steach, Holly R.; Fitzpatrick, Kristin S.; Gewe, Mesfin M.; Skog, Patrick D.; Doyle-Cooper, Colleen; Ota, Takayuki; Strong, Roland K.; Nemazee, David; Pancera, Marie; Stamatatos, Leonidas; McGuire, Andrew T.; Taylor, Justin J.

Atomic coordinates and structure factors of the IB2/iglb12 (6OL6) and IB3/iglb12 (6OL5) complex x-ray crystal structures have been deposited in the Protein Data Bank. The heavy and light chain sequences of IB1 (MK775682 and MK775686), IB2 (MK775683 and MK775687), IB3 (MK775684 and MK775688), and IB5 (MK775685 and MK775689) have been deposited in GenBank. Table S1 contains sequence information for the control antibodies used in Fig. 1 C. Table S2 contains sequence information for the heavy and light chain sequences derived from B cells binding the IB1/2/3 tetramer cocktail. Table S3 contains sequence information for the heavy and light chain sequences derived from human B cells binding the IB2 tetramers. Table S4 contains sequence information for the heavy and light chain sequences derived from human B cells binding the IB3 tetramers. Table S5 contains data collection and refinement statistics for the crystal structures shown in