Author: Lee, Hyojin; Kim, Eun-Ju; Song, Jae-Young; Choi, Jeong Soo; Lee, Ji Youn; Cho, In-Soo; Shin, Yeun-Kyung
Title: Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera Document date: 2016_9_20
ID: 0pe8vgin_33
Snippet: Tick-mediated SFTSV is an emerging infectious virus identified in 2009 that shows 12% lethality in humans [23] . However, the range of host species or virus circulation routes in the environment is difficult to determine because a standard diagnosis method is not available. Therefore, a simple and effective method of detecting SFTSV antibody is needed to identify potential hosts and develop anti-viral strategies. In an effort to detect SFTSV-spec.....
Document: Tick-mediated SFTSV is an emerging infectious virus identified in 2009 that shows 12% lethality in humans [23] . However, the range of host species or virus circulation routes in the environment is difficult to determine because a standard diagnosis method is not available. Therefore, a simple and effective method of detecting SFTSV antibody is needed to identify potential hosts and develop anti-viral strategies. In an effort to detect SFTSV-specific antibodies, in-house ELISA and double-antigen sandwich ELISA (DAS-ELISA) assays were developed [5, 24] . Specifically, DAS-ELISA was used to determine the seroprevalence of humans and animals in China. A multiplexed Luminex-based immunoassay method was also established for high-throughput detection [20] . These newly developed techniques are highly specific and sensitive, but are not commercially available, and some of these methods require special equipment for diagnosis.
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