Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera

Lee, Hyojin; Kim, Eun-Ju; Song, Jae-Young; Choi, Jeong Soo; Lee, Ji Youn; Cho, In-Soo; Shin, Yeun-Kyung

Here, we report the development of a competitive ELISA (cELISA) based on the competition of SFTSV-specific antibodies in the test serum with monoclonal antibodies (mAbs) against the NP of SFTSV. The NP recombinant protein is suitable for use as a diagnostic antigen as it is a highly immunogenic protein that has been shown to be expressed from the early stage of virus infection in other Bunyaviridae cases [10, 11, 23] . We applied this technique to field bovine sera and experimentally generated bovine antisera against SFTSV and investigated the correlation and consistency of this assay with IFA. The cELISA presented in this study can be used to detect antibodies against SFTSV in cattle.