Selected article for: "cdna clone and clone cdna"
Author: Almazán, Fernando; DeDiego, Marta L.; Sola, Isabel; Zuñiga, Sonia; Nieto-Torres, Jose L.; Marquez-Jurado, Silvia; Andrés, German; Enjuanes, Luis
Title: Engineering a Replication-Competent, Propagation-Defective Middle East Respiratory Syndrome Coronavirus as a Vaccine Candidate
  • Document date: 2013_9_10
    • ID: 14yfs4pa_37
    Snippet: Construction of a MERS-CoV cDNA clone lacking the structural E gene. The pBAC-MERS-⌬E, encoding a MERS-CoV lacking the E gene, was constructed from the full-length plasmid pBAC-MERS FL . To this end, the SanDI-RsrII DNA fragment (2,634 bp) from pBAC-MERS FL was exchanged with a chemically synthesized (Bio Basic, Inc.) SanDI-RsrII DNA fragment with a deletion from nucleotides 27,580 to 27,786 that included the TRS core sequence and the first 197.....
    Document: Construction of a MERS-CoV cDNA clone lacking the structural E gene. The pBAC-MERS-⌬E, encoding a MERS-CoV lacking the E gene, was constructed from the full-length plasmid pBAC-MERS FL . To this end, the SanDI-RsrII DNA fragment (2,634 bp) from pBAC-MERS FL was exchanged with a chemically synthesized (Bio Basic, Inc.) SanDI-RsrII DNA fragment with a deletion from nucleotides 27,580 to 27,786 that included the TRS core sequence and the first 197 nucleotides of the E gene (Fig. 4A) . The genetic integrity of the cloned DNA was verified by restriction analysis and sequencing.

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