Author: Mohammadzadeh, Sara; Khabiri, Alireza; Roohvand, Farzin; Memarnejadian, Arash; Salmanian, Ali Hatef; Ajdary, Soheila; Ehsani, Parastoo
Title: Enhanced-Transient Expression of Hepatitis C Virus Core Protein in Nicotiana tabacum, a Protein With Potential Clinical Applications Document date: 2014_11_24
ID: 3posyr5n_12
Snippet: The infiltrated tobacco leaves were ground into a fine powder in liquid nitrogen, using mortar and pestle. Subsequently, 0.5-mL extraction buffer (50 mM NaH 2 PO 4 , pH = 8, 300 mM NaCl, 0.1% Tween 20, 10 mM beta-mercaptoethanol, 1 mM PMSF, and 5 mM EDTA) was added to each gram of powdered leaf tissue. The total soluble protein (TSP) was removed from cell debris by centrifugation of the leaf extracts for 20 minutes at 4°C (14000 rpm)......
Document: The infiltrated tobacco leaves were ground into a fine powder in liquid nitrogen, using mortar and pestle. Subsequently, 0.5-mL extraction buffer (50 mM NaH 2 PO 4 , pH = 8, 300 mM NaCl, 0.1% Tween 20, 10 mM beta-mercaptoethanol, 1 mM PMSF, and 5 mM EDTA) was added to each gram of powdered leaf tissue. The total soluble protein (TSP) was removed from cell debris by centrifugation of the leaf extracts for 20 minutes at 4°C (14000 rpm).
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