Establishment of minimal positive-control conditions to ensure brain safety during rapid development of emergency vaccines

Baek, Hyekyung; Kim, Kwang Ho; Park, Min Young; Kim, Kyeongryun; Ko, Bokyeong; Seo, Hyung Seok; Kim, Byoung Soo; Hahn, Tae-Wook; Yi, Sun Shin

Western blot results showed that ZO-1 and occludin protein expression tendencies were similar to the mRNA results in both mouse strains. In B6 mice, there were small changes in ZO-1 and occludin expression levels between 0 and 24 h PI, but the changes were not significant. Interestingly, ZO-1 protein expression in ICR mice continuously and time-dependently increased during the 24 h after LPS(S) injection (panels A and B in Fig. 3 ); in contrast, the ZO-1 mRNA level at 24 h PI had decreased to a level similar to that at injection (panel A in Fig. 2 ). Occludin protein expression in ICR mice showed a temporal pattern that was similar to that for occludin mRNA expression. The occludin proteins in B6 mice did not change significantly, but, in ICR mice, there was a significant increase in occludin protein expression but only at 24 h PI, which was similar to the occludin mRNA level result in ICR mice (panel C in Fig. 3) . The results of mRNA and protein levels have correlation each other and putative mechanisms of LPS-inducing reaction were showed (panels A and B in Fig. 4) . The different tendencies of ZO-1 and occludin mRNA and protein expressions in ICR mice are also summarized with the stream graphs (panel C in Fig. 4) .