Author: Dong, Xiaoxv; Ni, Boran; Fu, Jing; Yin, Xingbin; You, Longtai; Leng, Xin; Liang, Xiao; Ni, Jian
Title: Emodin induces apoptosis in human hepatocellular carcinoma HepaRG cells via the mitochondrial caspase-dependent pathway Document date: 2018_8_2
ID: 02r8i5n1_23
Snippet: To explore the possible mechanisms of emodin-induced apoptosis in HepaRG cells, we evaluated the expression of various proteins involved in the mitochondrial signaling pathway by Western blot analysis. We evaluated the expression of Bcl-2 family associated proteins and caspases, which are involved in the mitochondrial signaling pathway. Treatment with emodin significantly increased the expression of pro-apoptotic Bax and decreased the expression .....
Document: To explore the possible mechanisms of emodin-induced apoptosis in HepaRG cells, we evaluated the expression of various proteins involved in the mitochondrial signaling pathway by Western blot analysis. We evaluated the expression of Bcl-2 family associated proteins and caspases, which are involved in the mitochondrial signaling pathway. Treatment with emodin significantly increased the expression of pro-apoptotic Bax and decreased the expression of anti-apoptotic Bcl-2 in a dose-dependent manner, thereby significantly resulting in an elevated Bax/Bcl-2 ratio. Moreover, caspases are the most important effector molecules for the execution of apoptosis. Compared with the vehicle-treated control group, emodin treatment obviously enhanced the expression of cleaved caspase-3, -9, Bax and PARP (Fig. 7A and B) .
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