Author: Poe, Jonathan C.; Kountikov, Evgueni I.; Lykken, Jacquelyn M.; Natarajan, Abirami; Marchuk, Douglas A.; Tedder, Thomas F.
Title: EndoU is a novel regulator of AICD during peripheral B cell selection Document date: 2014_1_13
ID: 5804sjmo_19
Snippet: Because c-Myc expression is reduced in CD22 /[B6] and Ig Tg sHEL B cells after BCR ligation and c-Myc transcripts contain numerous stretches of poly(U) residues within the 3 untranslated region (3-UTR, Fig. 3 F) , c-Myc mRNA interactions with EndoU were examined. Like the XendoU ssRNA substrate, c-Myc mRNA was also bound by recombinant EndoU (Fig. 3 F) , but mRNA cleavage was not detectable (not depicted). Thus, c-Myc mRNA is a potent.....
Document: Because c-Myc expression is reduced in CD22 /[B6] and Ig Tg sHEL B cells after BCR ligation and c-Myc transcripts contain numerous stretches of poly(U) residues within the 3 untranslated region (3-UTR, Fig. 3 F) , c-Myc mRNA interactions with EndoU were examined. Like the XendoU ssRNA substrate, c-Myc mRNA was also bound by recombinant EndoU (Fig. 3 F) , but mRNA cleavage was not detectable (not depicted). Thus, c-Myc mRNA is a potential EndoU substrate, which may explain reduced c-Myc expression when B cells overexpress EndoU. Further supporting this notion, ectopic EndoU expression in mouse NIH-3T3 fibroblasts significantly reduced c-Myc protein levels (Fig. 3 G; 32% decrease, P < 0.05).
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