Selected article for: "flow cytometry and surface expression"
Author: Poe, Jonathan C.; Kountikov, Evgueni I.; Lykken, Jacquelyn M.; Natarajan, Abirami; Marchuk, Douglas A.; Tedder, Thomas F.
Title: EndoU is a novel regulator of AICD during peripheral B cell selection
  • Document date: 2014_1_13
    • ID: 5804sjmo_47
    Snippet: Adoptive transfer experiments. Purified spleen B cells (15 × 10 6 ) from high-responder EndoU / Ig Tg sHEL mice were labeled with eFluor 670 cell tracking dye (eBioscience) and injected intraperitoneally in sterile PBS into WT or sHEL recipient mice. After 36 h, peritoneal cavity and spleen leukocytes were harvested and analyzed by flow cytometry for the presence of the adoptively transferred B cells along with assessment of their IgM a su.....
    Document: Adoptive transfer experiments. Purified spleen B cells (15 × 10 6 ) from high-responder EndoU / Ig Tg sHEL mice were labeled with eFluor 670 cell tracking dye (eBioscience) and injected intraperitoneally in sterile PBS into WT or sHEL recipient mice. After 36 h, peritoneal cavity and spleen leukocytes were harvested and analyzed by flow cytometry for the presence of the adoptively transferred B cells along with assessment of their IgM a surface expression levels and HEL-binding capacity.

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