Establishment of minimal positive-control conditions to ensure brain safety during rapid development of emergency vaccines

Baek, Hyekyung; Kim, Kwang Ho; Park, Min Young; Kim, Kyeongryun; Ko, Bokyeong; Seo, Hyung Seok; Kim, Byoung Soo; Hahn, Tae-Wook; Yi, Sun Shin

Even though some previous in vitro studies have shown that TJ mRNA and protein levels decrease after LPS injection [21, 25] , a compensatory mechanism could not be generally achieved in vitro than in vivo. In other words, even though the BBB structures were destroyed and its function was impaired, it could not be recovered in vitro system in plastic plate or flask, because there are no other kinds of cells or tissues which counteract and cooperate with epithelial cells with TJs. However, in vivo system, the compensatory reaction in the body could be activated by various cells and tissues for healing of impaired region. Therefore, we believe that the expressions of TJ-related mRNAs and proteins continuously increase until TJ structure and function are restored through physiological processes in animal models. In this study, B6 mice showed lower BBB penetrance of EB dye than that in ICR mice; moreover, in both mouse strains, the mRNA and protein expression results from both mice strains generally corresponded with EB assay results.