Author: Mok, Hoyin; Cheng, Xing; Xu, Qi; Zengel, James R; Parhy, Bandita; Zhao, Jackie; Wang, C. Kathy; Jin, Hong
Title: Evaluation of Measles Vaccine Virus as a Vector to Deliver Respiratory Syncytial Virus Fusion Protein or Epstein-Barr Virus Glycoprotein gp350 Document date: 2012_2_16
ID: 3qdjmb2j_14
Snippet: Cotton rat studies. Specific-pathogen-free 6-to 8-weekold cotton rats were purchased from Harlan (Indianapolis, IN). The animal study protocol was approved by MedImmune's Institutional Animal Care and Use Committee. Animals were anesthetized with isoflurane by inhalation and vaccinated intramuscularly (i.m.) with 10 5 PFU of rEZ, gp350tr, gp350, sF1 or sF3 in a 200 μl inoculum volume. The control groups were inoculated intramuscularly (i.m) with.....
Document: Cotton rat studies. Specific-pathogen-free 6-to 8-weekold cotton rats were purchased from Harlan (Indianapolis, IN). The animal study protocol was approved by MedImmune's Institutional Animal Care and Use Committee. Animals were anesthetized with isoflurane by inhalation and vaccinated intramuscularly (i.m.) with 10 5 PFU of rEZ, gp350tr, gp350, sF1 or sF3 in a 200 μl inoculum volume. The control groups were inoculated intramuscularly (i.m) with PBS, 10 5 PFU of UV-inactivated sF3, or intransally (i.n) with 10 6 PFU of RSV A2 strain. The animals were boosted with the same dose of viruses 4 weeks later.
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