Author: Won, Hokeun; Lee, Dong-Uk; Jang, Guehwan; Noh, Yun-Hee; Lee, Seung-Chul; Choi, Hwan-Won; Yoon, In-Joong; Yoo, Han Sang; Lee, Changhee
Title: Generation and protective efficacy of a cold-adapted attenuated genotype 2b porcine epidemic diarrhea virus Document date: 2019_7_9
ID: 2hxlx1j2_10
Snippet: Vero cells were infected with each Aram virus stock in the presence of trypsin as described above. The culture supernatants were collected 48 h post-infection (hpi) when 70% CPE commonly developed. Virus titers were measured by end-point titration in 96-well plates using 10-fold serial dilutions of the supernatant samples in triplicate for each dilution to determine the amount of virus required to produce CPE in 50% of the inoculated Vero cells. .....
Document: Vero cells were infected with each Aram virus stock in the presence of trypsin as described above. The culture supernatants were collected 48 h post-infection (hpi) when 70% CPE commonly developed. Virus titers were measured by end-point titration in 96-well plates using 10-fold serial dilutions of the supernatant samples in triplicate for each dilution to determine the amount of virus required to produce CPE in 50% of the inoculated Vero cells. The 50% tissue culture infectious dose (TCID 50 ) per mL of virus stock was calculated using the Reed and Muench method [25] .
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