Author: OHTANI, Akifumi; KUBO, Masahito; SHIMODA, Hiroshi; OHYA, Kenji; IRIBE, Tadashi; OHISHI, Daiki; ENDOH, Daiji; OMATSU, Tsutomu; MIZUTANI, Tetsuya; FUKUSHI, Hideto; MAEDA, Ken
Title: Genetic and antigenic analysis of Chlamydia pecorum strains isolated from calves with diarrhea Document date: 2015_2_27
ID: 4itsd2aq_8
Snippet: Rabbit antisera described above were used for the crossneutralization test. Sera were serially diluted twofold in MEM in 96-well microplates. Each dilution was mixed with an equal volume of 200 TCID 50 /0.05 ml of C. pecorum strains and incubated at 37°C for 1 hr. Then, 0.1 ml (approximately 6 × 10 3 cells) of HmLu-1 cells in MEM containing 10% fetal bovine serum was added to each well. After incubation at 37°C for 7 days, antibody titers were.....
Document: Rabbit antisera described above were used for the crossneutralization test. Sera were serially diluted twofold in MEM in 96-well microplates. Each dilution was mixed with an equal volume of 200 TCID 50 /0.05 ml of C. pecorum strains and incubated at 37°C for 1 hr. Then, 0.1 ml (approximately 6 × 10 3 cells) of HmLu-1 cells in MEM containing 10% fetal bovine serum was added to each well. After incubation at 37°C for 7 days, antibody titers were expressed as the reciprocal of the highest dilution of serum which inhibited CPE completely.
Search related documents:
Co phrase search for related documents- antibody titer and fetal bovine: 1, 2
- antibody titer and fetal bovine serum: 1, 2
- antibody titer and high dilution: 1
- equal volume and fetal bovine: 1, 2, 3
- equal volume and fetal bovine serum: 1, 2, 3
- equal volume and Rabbit antisera: 1, 2
- fetal bovine and MEM cell: 1
- fetal bovine serum and MEM cell: 1
Co phrase search for related documents, hyperlinks ordered by date