Selected article for: "growth phase and haemolytica culture"
Author: Tucci, Paula; Estevez, Verónica; Becco, Lorena; Cabrera-Cabrera, Florencia; Grotiuz, Germán; Reolon, Eduardo; Marín, Mónica
Title: Identification of Leukotoxin and other vaccine candidate proteins in a Mannheimia haemolytica commercial antigen
  • Document date: 2016_9_19
    • ID: 71omypkg_42
    Snippet: Based on previous reports that detected Leukotoxin A activity in bacterium-free culture supernatant from logarithmic-phase cultures (Chang et al., 1987) , Neumosan line of vaccines includes inactivated culture supernatants of an industrial optimized M. haemolytica culture. In this work we confirmed that LKT secretion is linked to the exponential growth phase during the antigen production culture, representing one of the primary secreted component.....
    Document: Based on previous reports that detected Leukotoxin A activity in bacterium-free culture supernatant from logarithmic-phase cultures (Chang et al., 1987) , Neumosan line of vaccines includes inactivated culture supernatants of an industrial optimized M. haemolytica culture. In this work we confirmed that LKT secretion is linked to the exponential growth phase during the antigen production culture, representing one of the primary secreted components. In culture supernatant, LKT was detected as a single band with the expected MW (approx. 110 kDa) (Lo et al., 1985; Chang et al., 1987) . Interestingly, after formaldehyde treatment, LKT was detected as a higher MW band (>230 kDa),

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