Selected article for: "sds polyacrylamide and total protein"
Author: Jorge Blazquez-Prieto; Covadonga Huidobro; Ines Lopez-Alonso; Laura Amado-Rodriguez; Paula Martin-Vicente; Cecilia Lopez-Martinez; Irene Crespo; Cristina Pantoja; Pablo J Fernandez-Marcos; Manuel Serrano; Jacob I Sznajder; Guillermo M Albaiceta
Title: Cellular senescence limits acute lung injury induced by mechanical ventilation
  • Document date: 2020_3_25
    • ID: ebwxryai_30
    Snippet: Nuclei were extracted from fresh lung tissue or cultured cells and subsequently homogenized as described before (8) . The total amount of protein from nuclear extracts was quantified (BCA Protein Assay Kit, Pierce) and 15µg of each sample were loaded in SDS-polyacrylamide gels, electrophoresed at 120mV and electrotransferred onto PVDF membranes. After blockade with 5% non-fat dry milk, the membranes were incubated with primary antibodies against.....
    Document: Nuclei were extracted from fresh lung tissue or cultured cells and subsequently homogenized as described before (8) . The total amount of protein from nuclear extracts was quantified (BCA Protein Assay Kit, Pierce) and 15µg of each sample were loaded in SDS-polyacrylamide gels, electrophoresed at 120mV and electrotransferred onto PVDF membranes. After blockade with 5% non-fat dry milk, the membranes were incubated with primary antibodies against Lamin-A/C, Lamin-B1, gH2AX, HP1a or H3 (Supplementary Table 3 ) in 3% non-fat dry milk overnight at 4°C. After 24 hours, the membranes were incubated with the corresponding peroxidase-conjugated secondary antibodies in 2.5% non-fat dry milk. Proteins were detected by chemiluminescence in a LAS-4000 Imaging system. The intensity of each protein band was quantified using ImageJ software (NIH, USA).

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