Author: Liu, Hong Yan; Gao, Xiaohu
Title: A Universal Protein Tag for Delivery of SiRNA-Aptamer Chimeras Document date: 2013_11_7
ID: 0atfsivf_25
Snippet: Characterization of RNA binding capability of the four protein tags. The binding capabilities of the four polyhistidine modified dsRBD proteins were evaluated by native agarose gel. The chimera was labeled with FAM at the 59 end of siRNA's sense strand (IDT). To prepare chimera/dsRBD complex, chimera (5 mM, 10 ml) was incubated with the protein tags at protein/chimera molar ratios of 1, 2, or 4 for 1 h at 4uC. Bound chimera and unbound chimera we.....
Document: Characterization of RNA binding capability of the four protein tags. The binding capabilities of the four polyhistidine modified dsRBD proteins were evaluated by native agarose gel. The chimera was labeled with FAM at the 59 end of siRNA's sense strand (IDT). To prepare chimera/dsRBD complex, chimera (5 mM, 10 ml) was incubated with the protein tags at protein/chimera molar ratios of 1, 2, or 4 for 1 h at 4uC. Bound chimera and unbound chimera were quantified on 1% agarose gel using a Macro imaging system (Lightools Research, CA).
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