Author: Ivanova, Elena; Berger, Audrey; Scherrer, Anne; Alkalaeva, Elena; Strub, Katharina
Title: Alu RNA regulates the cellular pool of active ribosomes by targeted delivery of SRP9/14 to 40S subunits Document date: 2015_3_11
ID: 64cnoqpi_24
Snippet: To examine the effects of AluY A and scAluY A RNPs on translation we used a rabbit reticulocyte lysate (RRL) programmed with bovine preprolactin (pPL) mRNA. Addition of either AluY A or scAluY A RNPs diminished the translation efficiencies of pPL mRNA in a similar, dose-dependent manner whereas the addition of RNA or protein alone had no effect ( Figure 1B ). Translation efficiency was reduced to 50% in the presence of 100 nM AluY A or scAluY A R.....
Document: To examine the effects of AluY A and scAluY A RNPs on translation we used a rabbit reticulocyte lysate (RRL) programmed with bovine preprolactin (pPL) mRNA. Addition of either AluY A or scAluY A RNPs diminished the translation efficiencies of pPL mRNA in a similar, dose-dependent manner whereas the addition of RNA or protein alone had no effect ( Figure 1B ). Translation efficiency was reduced to 50% in the presence of 100 nM AluY A or scAluY A RNPs and, surprisingly, remained unchanged up to 5-fold higher RNP concentrations indicating that the inhibitory effect reached saturation.
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