Selected article for: "clinical outcome and CSF sample"
Title: 2016 ACVIM Forum Research Abstract Program
  • Document date: 2016_5_31
    • ID: 2y1y8jpx_126
    Snippet: CSF samples were contaminated with incremental amount of whole blood via serial dilution and LD and CK total and isoenzyme activity values were compared to an uncontaminated CSF sample and the established reference interval. Total LD and CK values were determined spectrophotometrically using a Sieman Dimension Xpand and isoenzyme activity was determined using gel electrophoresis and densitometric scanning. In contaminated samples, total LD and CK.....
    Document: CSF samples were contaminated with incremental amount of whole blood via serial dilution and LD and CK total and isoenzyme activity values were compared to an uncontaminated CSF sample and the established reference interval. Total LD and CK values were determined spectrophotometrically using a Sieman Dimension Xpand and isoenzyme activity was determined using gel electrophoresis and densitometric scanning. In contaminated samples, total LD and CK values were increased compared to the uncontaminated sample after contamination with > 2000 and > 4000 RBC/ul, respectively. Additionally, CSF isoenzyme activity began to emulate serum isoenzyme activity, where CK-BB and LD-5 banding became more prominent, when CSF was contaminated with > 4000 RBC/ul. These findings suggest there is a threshold where blood contamination can artificially distort CSF LD and CK total and isoenzyme analysis. Therefore, these values will need to be interpreted with caution when there is iatrogenic blood contamination in CSF. The objective of the study was to compare the clinical outcome between dogs with acute non-compressive nucleus pulposus extrusion (ANNPE) or presumptive fibrocartilaginous embolic myelopathy (FCEM) affecting the thoracolumbar spinal cord segments.

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