2016 ACVIM Forum Research Abstract Program

In conclusion, the human granulocyte immunofluorescence assay used in this study was able to identify sero-positivity for ANCA in a high percentage of dogs with FRD. The data suggests that this assay could be used as an alternative to the canine assay for detection of ANCA in dogs. Citrulline, a non-proteinogenic amino acid, has recently been described as a biomarker of enterocyte function in humans. Decreased concentrations of plasma citrulline have been reported in patients with short bowel syndrome, Crohn's disease, viral enteritis, or in critically ill patients. In dogs, the relationship between serum citrulline concentration and small intestinal dysfunction has not been well characterized. Citrulline is mainly synthesized from glutamine in enterocytes and is metabolized in the duodenum and jejunum. Therefore, intestinal injury can lead to decreased serum citrulline concentrations. The aims of this study were to analytically validate a GC/MS method for the measurement of citrulline in canine serum and to assess the concentration of this amino acid in dogs with chronic enteropathy.