Selected article for: "Extraction kit and Nucleic acid"
Author: Timurkan, Mehmet Ozkan; Aydin, Hakan; Sait, Ahmet
Title: Identification and Molecular Characterisation of Bovine Parainfluenza Virus-3 and Bovine Respiratory Syncytial Virus - First Report from Turkey
  • Document date: 2019_6_12
    • ID: 2sm0pgt7_11
    Snippet: Extraction of the viral nucleic acid. Nasal swabs were vortexed in 1 mL of PBS and centrifuged at 3,000 rpm for 5 min. Supernatant was collected and 200 µL was used for extraction. A total of 25 mg of each tissue sample was homogenised in 500 µL PBS using a Tissue Lyser II homogenizer (Qiagen, Germany) and centrifuged at 3,000 rpm at room temperature, and then 200 µL of the supernatant was used for extraction. A GF-1 viral nucleic acid kit (Vi.....
    Document: Extraction of the viral nucleic acid. Nasal swabs were vortexed in 1 mL of PBS and centrifuged at 3,000 rpm for 5 min. Supernatant was collected and 200 µL was used for extraction. A total of 25 mg of each tissue sample was homogenised in 500 µL PBS using a Tissue Lyser II homogenizer (Qiagen, Germany) and centrifuged at 3,000 rpm at room temperature, and then 200 µL of the supernatant was used for extraction. A GF-1 viral nucleic acid kit (Vivantis, Malaysia) was used for nucleic acid extraction according to the manufacturer's recommendations. All extracts were kept at −20°C before testing.

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