Selected article for: "negative control and siRNA transfection"

Author: Yen, Wei-Chen; Wu, Yi-Hsuan; Wu, Chih-Ching; Lin, Hsin-Ru; Stern, Arnold; Chen, Shih-Hsiang; Shu, Jwu-Ching; Tsun-Yee Chiu, Daniel
Title: Impaired inflammasome activation and bacterial clearance in G6PD deficiency due to defective NOX/p38 MAPK/AP-1 redox signaling
  • Document date: 2019_11_2
  • ID: 6fw4thkq_12
    Snippet: Specific sets of human siRNA for G6PD and a universal negative control were obtained from Dharmacon RNA Technologies (Lafayette, CO, USA). Transfection of the target siRNA (50 nM per 10 6 cells) was performed by using Lipofectamine 3000 reagent (Invitrogen, CA, USA) based on the manufacturer's instructions. On the next day, the cells were treated with stimuli as described below......
    Document: Specific sets of human siRNA for G6PD and a universal negative control were obtained from Dharmacon RNA Technologies (Lafayette, CO, USA). Transfection of the target siRNA (50 nM per 10 6 cells) was performed by using Lipofectamine 3000 reagent (Invitrogen, CA, USA) based on the manufacturer's instructions. On the next day, the cells were treated with stimuli as described below.

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