Impaired inflammasome activation and bacterial clearance in G6PD deficiency due to defective NOX/p38 MAPK/AP-1 redox signaling

Yen, Wei-Chen; Wu, Yi-Hsuan; Wu, Chih-Ching; Lin, Hsin-Ru; Stern, Arnold; Chen, Shih-Hsiang; Shu, Jwu-Ching; Tsun-Yee Chiu, Daniel

G6PD-kd THP-1 cells expressed approximately 21.7% of the G6PD levels in the control THP-1 cells (Fig. 2A) . According to the results of MTT assay and the Cell Counting Kit-8 (CCK8), cell viability was similar between control and G6PD-kd THP-1 cells (Fig. S1 ). Similar to that observed in PBMCs from G6PD-deficient patients (Fig. 1B) , G6PD-kd THP-1 cells had decreased IL-1β secretion upon inflammasome activation (Fig. 2B) , and the pattern of protein expression in the cell lysates and supernatants was the same as that found by ELISA (Fig. 2C) . The quantitative intensity of IL-1β in the cell lysate and supernatant is shown in Fig. 2D and E, respectively. The activity of G6PD was positively correlated with the secretion of IL-1β (Fig. 2F ).